Studies on Phytoconstituents and In-vitro Anti-Sickling and Polymerisation Inhibition Potentials of Chloroform, Ethyl Acetate and n-Butanol Partitioned Portions of the Crude Methanol Leaf Extract of Terminalia catappa Linn.

Authors

  • Ali Mohammed Fulata Federal Polytechnic Monguno

Abstract

 The results revealed that the partitioned portions of the crude methanol leaf extracts of Terminalia catappa revealed the presence of terpenoids and cardiac glycosides in the chloroform partitioned portion, tannins, cardiac glycoside, flavonoids, terpenoids and saponins in ethylacetate and n-butanol partitioned portions respectively. All the partitioned portions are devoid of alkaloids and phlabotannins. The results of the sickling reversal and inhibition potentials of the partitioned portions of Terminalia catappa methanol leaf extract revealed that the two partitioned portions of ethyl-acetate and n-butanol have effectively inhibited and reversed sickled red blood cells. The ethyl-acetate partitioned portion treatment groups provided significant protection and acted in dose dependent manner. Thus, reducing the percentage sickled cells to 18.61% at 12.5 mg/ml and 9.55% at 25 mg/ml corresponding to 63.73% and 81.39% percentage inhibition. Likewise, the n-butanol partitioned portion provided the most significant inhibitory activity with percentage sickling values of 3.29% and 2.24% corresponding to percentage inhibition values of 93.59% at 12.5 mg/ml and 95.63% 25 mg/ml respectively. Similarly, for the sickling reversal the ethylacetate partitioned portions acted in non-dose dependent manner with percentage sickling of 16.85 and 20.06% corresponding to 59.25% and 51.49% reversals respectively. However, the n-butanol partitioned portions revealed at 6.76% and 2.40% sickling after treatment. Thus, corresponding to 83.65% and 94.20% reversals at 12.5 mg/ml and 25 mg/ml concentrations. The results of the polymerization inhibition potentials of the partitioned portions revealed that the ethyl acetate and n-butanol partitioned portions of T. catappa leaf extracts were very potent anti-sickling agents capable of inhibiting gelation to a very high levels, with ethyl acetate portion having the relative percentage of inhibitions of 98.17% at 12 mg/ml and 92.65% at 25 mg/ml. While, n-butanol have inhibitory values of 98.97 at 12 mg/ml and 96.79 at 25 mg/ml.

References

Aimola, I. A., Inuwa, H. M., Nok, A. J. and Mamman, A. I. (2014). Induction of Fetal Haemoglobin Synthesis in Erythroid Progenitor Stem Cells: Mediated by Water‑Soluble Components of Terminalia catappa. Cell Biochemistry Function, 32:361‑367.Burkil, H. M. (1997). The useful plants of West Tropical Africa, Royal Botanical Gardens families, kew, U.K, 4: 969Chen, P. S. and Li, J. H. (2006). Chemopreventive Effect of Punicalagin, a Novel Tannin Component Isolated from Terminaliacatappa, on H-ras-transformed NIH3t3 Cells. Toxicology letters, 163 (1):44-53.Chitmanat, C., Tongdonmuan, K., Khanom, P., Pachontis, P. and Numsong, W. (2005). Antiparasitic, Antibacterial and Antifungal Activities Derived from a Terminaliacatappa Solution Against Some Tilapia oreochromisniloticus pathogens. Acta Horticulturae, 678: 179-182.Crozier, A., Burns, J., Aziz, A. A., Stewart, A. J., Jenkins, G. I. and Lean, M. E. J. (2000). Antioxidant flavonoids from fruits, vegetables and beverages; measurements and bioavailability. Biol. Res., 33:79-88.Cyril-Olutayo, M. C., Temitope, A. A., Ayodeji, O. O. and Joseph, M. A. (2020). Bioactivity-Directed Isolation of Anti-Sickling Compounds from Cnidoscolusacontifolius (Mill.) I.M. Johnst leaf extract. Journal of Pharmacy and Pharmacognosy Research, 8(6): 580-590. Cyril-Olutayo, M.C., Agbedahunsi, J. M. and Akinola, N. O. (2018). In vitro Evaluation of Moringa oleifera Leaf Extracts Used in Managing Sickle Cell Patients in South West Nigeria.Nigerian Journal of Pharmaceutical Research.14 (1):69-79.Dan’azumi, U., Fulata, A. M., Suleman A., Mohammed, A., Garandawa, M. M. (2019). Preliminary Phytochemical Screening and GC-MS Analysis of the Bioactive Components of the Leaf of Terminalia catappa. International Journal of Research and Innovation in Applied Science (IJRIAS) 3(12): 29-45.Fall, A. B., Fastré, R. V., Vanhaelen, M., Lo, I., Toppet, M., Ferster, A. and Fondu, P. (1999). In-vitro anti-sickling activity of a rearranged limonoid isolated from Khayasenegalensis. PlantaMedica., 65(3):209–212.Fulata, A. M.,Usman, H., Tijjani, M. A., Daja, A. and Bukar, A. (2022). Phytochemical, anti-sickling and polymerization inhibition studies of the crude methanol leaf recipe extracts of three medicinal plants. International Journal of Advanced Chemistry Research ,4(1): 42-47Harbone, J. B. (1973). Phytochemical Methods. Champman and Hull Ltd, London. pp 49 – 188.Ibegbulem, C.O., Eyong, E.U. and Essien, E.U. (2011). Biochemical Effects of Drinking Terminaliacatappa Linn, Decoction in Wister Rats. African Journal of Biochemical Research, 5:237-243.Khan, A. A., Kumar, V., Singh, B. K. and Singh, R. (2014). Evaluation of Wound Healing Property of Terminalia catappa on Excision Wound Models in Wister Rats. Drugs Research (stuttg), 64:225-228.Mishra, P. K., Sharma, S., Jain, V., Tiwara, J., Mishra, M., Patra, P. K. and Khodiar, P. K. (2018). Antisickling and antioxidant relevance of twelve ethnomedicinal plants. Medicinal Plants. 10(3): 226-235.Mishra, S., Shruti, S., Manish, K. D. and Prashant, K. S. (2022). Anti sickling potential and chemical profiling of traditionally used Woodfordiafruticosa(L.) Kurzleaves. Arabian Journal of Chemistry,15: 1-15Mpiana, P. T., Mudogo, V., Ngbolua, K. N., Tshibangu, D. S. T., Shetonde, O. M. and Mbala, M.B. (2007) In Vitro Antisickling Activity of Anthocyanins from Ocimum basilicumL. (Lamiaceae). International Journal of Pharmacology,3: 371-4 Mpiana, P.T., Misakabu, F.M., Tshibangu, D.S.T., Ngbolua, K.N. and Mwanangombo, D.T. (2014) Antisickling Activity and Membrane Stabilization Effect of Anthocyanins Extracts from Adansonia digitata L. Barks on Sickle Blood Cells. International Blood Research and Reviews, 2:198-212Muhammad, A. and Mudi, S. Y. (2011). Phytochemical Screening and Antimicrobial Activities of Terminalia catappa Leaf Extracts. Biokemestri, 23(1): 35-39.Mustapha, A. T., Mohammed, G. T. and Abdulrahman, F.I. (2020). Chemical Composition and Radical scavenging (antioxidant) Efficacy of the Leaf of Terminalia catappa Linn. Extended Abstract. Journal of Clinical and Experimental Toxicology, 4(3):1-2Nurain, I. O., Bewaji, C. O., Johnson, J. S., Robertson, D. D. and Zhang, Y. (2017). Potential of Three Ethnomedicinal Plants as Anti-Sickling Agents. Molecular pharmaceutics, 14: 172−182. Nwaoguikpe, R. N., Ujowundu, C. O. and Okwu, G. N. (2013). The Anti-Sickling Potentials of Four Curcubits (T. Occidentalis, C. Maxima; C. Sativus and C. Lonatus). Scholars Journal of Applied Medical Science, 1 (3): 191−198.Onah, J. O., Akubue, P. I. andOkide, G. B. (2002). The Kinetics of Reversal of Pre-Sickled Erythrocytes by the Aqueous Extract of Cajanus cajan Seeds, Phytotherapy Research, 16(8):748–750.Orjinta, U.M. (2018). In vitro antisickling properties of aqueous and ethanol leaf extracts of moringa oleifera. Unpublisheda thesis submitted to the postgraduate school Federal University of Technology, Owerri in partial fulfilment of the requirement for the award of the degree of master of science (M.Sc.) In biochemistry. p. 1-156.Osuagwu, C. G. (2010). Mechanism of the antisickling effects of Cajanus cajan andphenylalanine. Nigerian Journal of Biochemistry and Molecular Biology, 25(2): 68-71.Pauline, N., Cabral, B. N. P., Anatole, P. C., Jocelyne, A. M. V., Bruno, M. and Jeanne, N. Y. (2013). The in vitro Anti-Sickling and Antioxidant Effects of Aqueous Extracts Zanthoxyllum heitzii on sickle cell disorder. BMC Complementary Alternative Medicine, 13(1): 162.Pawar, S. P. and Pal, S. C. (2002). Antimicrobial Activity of Extracts of Terminalia catappa Root. Indian Journal of Medical Sciences, 56:276-278.Ratnasooriya, W. D., Dharmasiri, M. G., Rajapakse, R. A., De Silva, M. S., Jayawardena, S. P. and Fernando, P. U. (2002). Tender Leaf Extract of Terminalia catappa Antinociceptive Activity in Rats. Pharmacology and Biology, 40:60-66.Rees, D. C. and Gibson, J. S. (2012). Biomarkers in Sickle Cell Disease. British Journal of Haematology, 156: 433-45.Salinas, G. C. and Thein, S. L. (2021). Research in Sickle Cell Disease: From Bedside to Bench to Bedside Bidirectional Translational Research in Sickle Cell Disease Hemasphere 6(6): 1-10.Samuel, B. B., Olaniyi, A. A., Olakunle, I. D., Dambrosio, M. and Okogun, J. I. (2009). Phytochemical and Anti-Sickling Activities of Terminalia catappa Linn. JOPAT, 14:31- 36.Seidel, V. (2006). Initial and Bulk Extraction. In: Sarker, S. D., Latif, Z. and Gray, A. I. (eds.) Natural Products Isolation. New Jersey: Humana Press. p. 27-46.Silva, O., Duarte, A., Cabrita, J., Pimentel, M., Diniz, A. and Gomes, E. (1998). Antimicrobial Activity of Guinea-Bissau Traditional Remedies. Journal of Ethnopharmacology, 50: 55-59.Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa. Spectrum Books Ltd., Ibadan, Nigeria. pp 191-289.Trease, G. E. and Evans, W. C. (1989). Pharmacognosy, 11th Editon. BrailliarTridelCan.Macmillian Publishers London. pp 45-50.

Published on: 13-12-2024

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Fulata, A. M. (2024). Studies on Phytoconstituents and In-vitro Anti-Sickling and Polymerisation Inhibition Potentials of Chloroform, Ethyl Acetate and n-Butanol Partitioned Portions of the Crude Methanol Leaf Extract of Terminalia catappa Linn. Jabirian Journal of Biointerface Research in Pharmaceutics and Applied Chemistry, 1(5). Retrieved from https://sprinpub.com/jabirian/article/view/314

Issue

Vol. 1 No. 5 (2024): Volume 01 Issue 05

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Research Article

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